Coding

Part:BBa_K1811333:Design

Designed by: Samuel Keating   Group: iGEM15_UMBC-Maryland   (2015-08-26)


CUP1 metallothionein fused to LamB on outer membrane


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
    Illegal AgeI site found at 1399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon of CUP1 was removed before being inserted into LamB. In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3.


Source

LamB comes from NCBI ID 948548. CUP1 comes from NCBI ID 856450.

References